Negative Control for mouse IgG3, kappa Primary Antibody
- Catalog No.:VN0004
- Applications:IHC;IP;IF;ELISA
- Reactivity:Human;Mouse;Rat;Bovin;Pig;Chick;
- Human Swiss Prot No:
- /
- Specificity:
- 0
- Formulation:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source:
- Mouse, Monoclonal/IgG3, kappa
- Dilution:
- IHC 1:200-1000. IF 1:100-500. ELISA 1:1000-5000
- Purification:
- Protein G
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Subcellular Location:
- No staining
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- Products Images
- Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD42b mouse mAb (left) and mouse IgG3, kappa monoclonal control (VN004, right).
- Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 14 mouse mAb (YM6031, left) and mouse IgG3, kappa monoclonal control (VN004, right).
- GAPDH was immunoprecipitated from Hela whole cell lysate with anti-GAPDH (PTR2304) antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG (H + L) antibody. Lane 1 (Input) : Hela whole cell lysate Lane 2 (-) :Mouse monoclonal IgG3 (vn0004, Isotype Control) instead of GAPDH antibody in Hela whole cell lysate. Lane 3 (+) : GAPDH antibody IP in Hela whole cell lysate
- GAPDH was immunoprecipitated from SW480 whole cell lysate with anti-GAPDH (PTR2304) antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG (H + L) antibody. Lane 1 (Input) : SW480 whole cell lysate Lane 2 (-) : Mouse monoclonal IgG3 (vn0004, Isotype Control) instead of GAPDH antibody in SW480 whole cell lysate. Lane 3 (+) : GAPDH antibody IP in SW480 whole cell lysate