Total NEK9 Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA4194C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • NEK9
    • Human Swiss Prot No：
    • Q8TD19
    • Mouse Swiss Prot No：
    • Q8K1R7
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • Serine/threonine-protein kinase Nek9 (EC 2.7.11.1) (Nercc1 kinase) (Never in mitosis A-related kinase 9) (NimA-related protein kinase 9) (NimA-related kinase 8) (Nek8)
    • Detection Method：
    • Colorimetric
    • Background：
    • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,developmental stage:Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells.,domain:Dimerizes through its coiled-coil domain.,enzyme regulation:Activated during mitosis by intramolecular autophosphorylation. Activity and autophosphorylation is activated by manganese >> magnesium ions. Sensitive to increasing concentration of detergents. It is not cell-cycle regulated but activity is higher in G0-arrested cells.,function:Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.,PTM:Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDC2 in vitro.,similarity:Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 6 RCC1 repeats.,subunit:Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex.,tissue specificity:Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.,
    • Function：
    • M phase of mitotic cell cycle, mitotic cell cycle, M phase, nuclear division, protein amino acid phosphorylation,phosphorus metabolic process, phosphate metabolic process, cell cycle, mitosis, phosphorylation, cell cycle process,cell cycle phase, organelle fission, cell division,
    • Subcellular Location：
    • Cytoplasm . Nucleus .
    • Expression：
    • Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

    Attributes

    • Code：
    • KA4194C
    • Size
    • 96 / well
    • Price$：
    • 330
    • Delivery：
    • 2024-12-07
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