Total BLNK Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA4013C
  • Applications：ELISA
  • Reactivity：Human;Mouse
    • Gene Name：
    • BLNK
    • Human Swiss Prot No：
    • Q8WV28
    • Mouse Swiss Prot No：
    • Q9QUN3
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • B-cell linker protein (B-cell adapter containing a SH2 domain protein) (B-cell adapter containing a Src homology 2 domain protein) (Cytoplasmic adapter protein) (Src homology 2 domain-containing leukocyte protein of 65 kDa) (SLP-65)
    • Detection Method：
    • Colorimetric
    • Background：
    • disease:Defects in BLNK are the cause of hypoglobulinemia and absent B-cells [MIM:604515]. This is a developmental blockage at the pro- to pre-B-cell transition.,disease:In 6 of 34 childhood pre-B acute lymphoblastic leukemia (ALL) samples that were tested showed a complete loss or drastic reduction of BLNK expression.,function:Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.,online information:BLNK mutation db,PTM:Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.,similarity:Contains 1 SH2 domain.,subcellular location:BCR activation results in the translocation to membrane fraction.,subunit:Associates with PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with VAV3, PLCG2 and GRB2. Interacts through its SH2 domain with CD79A.,tissue specificity:Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.,
    • Function：
    • cell activation, immune system development, leukocyte differentiation, defense response, inflammatory response,immune response, humoral immune response, intracellular signaling cascade, response to wounding, hemopoiesis,lymphocyte differentiation, B cell differentiation, B cell activation, leukocyte activation, lymphocyte activation,hemopoietic or lymphoid organ development,
    • Subcellular Location：
    • Cytoplasm . Cell membrane . BCR activation results in the translocation to membrane fraction.
    • Expression：
    • Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.

    Attributes

    • Code：
    • KA4013C
    • Size
    • 96 / well
    • Price$：
    • 330
    • Delivery：
    • 2024-08-01
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