Total Midline-1 Cell-Based Colorimetric ELISA Kit
- Catalog No.:KA3457C
- Applications:ELISA
- Reactivity:Human;Mouse;Rat
- Gene Name:
- MID1
- Human Gene Id:
- 4281
- Human Swiss Prot No:
- O15344
- Mouse Swiss Prot No:
- O70583
- Rat Swiss Prot No:
- P82458
- Storage Stability:
- 2-8°C/6 months
- Other Name:
- Midline-1 (EC 6.3.2.-) (Midin) (Midline 1 RING finger protein) (Putative transcription factor XPRF) (RING finger protein 59) (Tripartite motif-containing protein 18)
- Detection Method:
- Colorimetric
- Background:
- disease:Defects in MID1 are the cause of Opitz syndrome type I (OS-I) [MIM:300000]. OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.,function:May have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation.,induction:A retroviral element acts as an alternative tissue-specific promoter for this gene. The LTR of an HERV-E element enhances the expression in placenta and embryonic kidney.,PTM:Phosphorylated on serine and threonine residues.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 1 COS domain.,similarity:Contains 1 fibronectin type-III domain.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 B box-type zinc fingers.,subcellular location:Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.,subunit:Homodimer or heterodimer with MID2. Interacts with IGBP1.,tissue specificity:In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.,
- Function:
- microtubule cytoskeleton organization, proteolysis, cytoskeleton organization, microtubule-based process, negative regulation of microtubule depolymerization, pattern specification process, macromolecule catabolic process, negative regulation of organelle organization, modification-dependent protein catabolic process, protein catabolic process,regulation of microtubule polymerization or depolymerization, negative regulation of microtubule polymerization or depolymerization, regulation of microtubule depolymerization, regulation of microtubule-based process, regulation of organelle organization, negative regulation of protein complex disassembly, regulation of protein complex disassembly, modification-dependent macromolecule catabolic process, cellular protein catabolic process, cellular macromolecule catabolic process, negative regulation of cellular component organization, regulation of
- Subcellular Location:
- Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, spindle . Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis.
- Expression:
- In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain.
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