Phospho SHP-2 (Y580) Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA1182C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • PTPN11
    • Human Gene Id：
    • 5781
    • Human Swiss Prot No：
    • Q06124
    • Mouse Swiss Prot No：
    • P35235
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • Tyrosine-protein phosphatase non-receptor type 11 (EC 3.1.3.48) (Protein-tyrosine phosphatase 1D) (PTP-1D) (Protein-tyrosine phosphatase 2C) (PTP-2C) (SH-PTP2) (SHP-2) (Shp2) (SH-PTP3)
    • Detection Method：
    • Colorimetric
    • Background：
    • catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,disease:Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.,disease:Defects in PTPN11 are a cause of Noonan-like syndrome [MIM:163955]; also known as Noonan-like/multiple giant cell lesion syndrome. It is an autosomal dominant disorder characterized by Noonan features associates with giant cell lesions of bone and soft tissue.,disease:Defects in PTPN11 are the cause of LEOPARD syndrome [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.,disease:Defects in PTPN11 are the cause of Noonan syndrome 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.,domain:The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.,function:Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.,PTM:Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,similarity:Contains 2 SH2 domains.,subunit:Interacts with phosphorylated LIME1 and BCAR3. Interacts with SHB and INPP5D/SHIP1 (By similarity). Interacts with PTPNS1 and CD84. Interacts with phosphorylated SIT1 and MPZL1. Interacts with FCRL3, FCRL4, FCRL6 and ANKHD1.,tissue specificity:Widely expressed, with highest levels in heart, brain, and skeletal muscle.,
    • Function：
    • cell cycle checkpoint, DNA damage checkpoint, MAPKKK cascade, activation of MAPK activity, cell morphogenesis, cell morphogenesis involved in differentiation, regulation of peptide secretion, negative regulation of peptide secretion,protein amino acid phosphorylation, protein amino acid dephosphorylation, neutral lipid metabolic process, acylglycerol metabolic process, triglyceride metabolic process, glycerol ether metabolic process, phosphorus metabolic process,phosphate metabolic process, response to DNA damage stimulus, cell surface receptor linked signal transduction,enzyme linked receptor protein signaling pathway, transmembrane receptor protein tyrosine kinase signaling pathway, intracellular signaling cascade, protein kinase cascade, axonogenesis, response to endogenous stimulus,response to hormone stimulus, hormone-mediated signaling, positive regulation of signal transduction, r
    • Subcellular Location：
    • Cytoplasm . Nucleus .
    • Expression：
    • Widely expressed, with highest levels in heart, brain, and skeletal muscle.

    Attributes

    • Code：
    • KA1182C
    • Size
    • 96 / well
    • Price$：
    • 470
    • Delivery：
    • 2024-08-03
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