- 靶点:
- SIN1
- 简介:
- >>mTOR signaling pathway
- 基因名称:
- MAPKAP1 MIP1 SIN1
- 蛋白名称:
- SIN1
- Human Gene Id:
- 79109
- Human Swiss Prot No:
- Q9BPZ7
- Mouse Gene Id:
- 227743
- Mouse Swiss Prot No:
- Q8BKH7
- Rat Gene Id:
- 296648
- Rat Swiss Prot No:
- Q6AYF1
- 免疫原:
- Synthesized peptide derived from human SIN1 AA range: 193-243
- 特异性:
- This antibody detects endogenous levels of SIN1 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 分子量:
- 57kD
- 背景:
- This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008],
- 功能:
- function:Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAs signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.,similarity:Belongs to the SIN1 family.,subunit:All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2.,tissue specificity:Ubiquitously expressed, with highest levels in heart and skeletal muscle.,
- 细胞定位:
- Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus.
- 组织表达:
- Ubiquitously expressed, with highest levels in heart and skeletal muscle.
SP1-Induced Upregulation of LncRNA AFAP1-AS1 Promotes Tumor Progression in Triple-Negative Breast Cancer by Regulating mTOR Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yidong Zhou WB Human 1:500 MDA-231 cell,MDA-MB-468 cell
货号:YT7258
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- 产品图片
- SP1-Induced Upregulation of LncRNA AFAP1-AS1 Promotes Tumor Progression in Triple-Negative Breast Cancer by Regulating mTOR Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yidong Zhou WB Human 1:500 MDA-231 cell,MDA-MB-468 cell
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).