• 靶点：
• Smad5

• 简介：
• >>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells

• 基因名称：
• SMAD5

• 蛋白名称：
• Mothers against decapentaplegic homolog 5

• Human Gene Id：
• 4090

• Human Swiss Prot No：
• Q99717

• Mouse Swiss Prot No：
• P97454

• Rat Gene Id：
• 59328

• Rat Swiss Prot No：
• Q9R1V3

• 免疫原：
• Synthesized peptide derived from the Internal region of human Smad5.

• 特异性：
• Smad5 Polyclonal Antibody detects endogenous levels of Smad5 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:40000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• SMAD5;MADH5;Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5

• 实测条带：
• 52kD

• 背景：
• The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],

• 功能：
• function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.,tissue specificity:Ubiquitous.,

• 细胞定位：
• Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

• 组织表达：
• Ubiquitous.

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