Wee 1 Polyclonal Antibody

    • 货号:YT4903
    • 应用:WB,ELISA
    • 种属:Human,Mouse,Rat
      • 基因名称:
      • WEE1
      • 蛋白名称:
      • Wee1-like protein kinase
      • Human Gene Id:
      • 7465
      • Human Swiss Prot No:
      • P30291
      • Mouse Swiss Prot No:
      • P47810
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human WEE1. AA range:19-68
      • 特异性:
      • Wee 1 Polyclonal Antibody detects endogenous levels of Wee 1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Rabbit
      • 稀释:
      • Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -20°C/1 year
      • 其他名称:
      • WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase
      • 分子量:
      • 71597
      • 实测条带:
      • 72
      • 背景:
      • WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],
      • 功能:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase
      • 细胞定位:
      • nucleus,nucleoplasm,nucleolus,cytoplasm,
      • 组织表达:
      • Amygdala,Blood,Epithelium,Human uterus endothel primary cell culture,Placenta,Skin,
      • 产品图片
      • Western blot analysis of lysates from 293 cells, using WEE1 Antibody. The lane on the right is blocked with the synthesized peptide.