Wee 1 Polyclonal Antibody
- 货号：YT4903
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Cell cycle;>>Human immunodeficiency virus 1 infection
- 蛋白名称：
- Wee1-like protein kinase
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human WEE1. AA range:19-68
- 特异性：
- Wee 1 Polyclonal Antibody detects endogenous levels of Wee 1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- WEE1;Wee1-like protein kinase;WEE1hu;Wee1A kinase
- 背景：
- WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase
- 组织表达：
- Amygdala,Blood,Epithelium,Human uterus endothel primary cell culture,Placenta,Skin,
- Western blot analysis of lysates from 293 cells, using WEE1 Antibody. The lane on the right is blocked with the synthesized peptide.
