Wee 1 (phospho Ser53) Polyclonal Antibody
- 货号:YP1064
- 应用:IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 靶点:
- WEE1
- 简介:
- >>Cell cycle;>>Human immunodeficiency virus 1 infection
- 基因名称:
- WEE1
- 蛋白名称:
- Wee1-like protein kinase
- Human Gene Id:
- 7465
- Human Swiss Prot No:
- P30291
- Mouse Gene Id:
- 22390
- Mouse Swiss Prot No:
- P47810
- Rat Gene Id:
- 308937
- Rat Swiss Prot No:
- Q63802
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser53. AA range:19-68
- 特异性:
- Phospho-Wee 1 (S53) Polyclonal Antibody detects endogenous levels of Wee 1 protein only when phosphorylated at S53.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- WEE1;Wee1-like protein kinase;WEE1hu;Wee1A kinase
- 分子量:
- 72kD
- 背景:
- WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 2 magnesium ions per subunit.,enzyme regulation:Synthesis is increased during S and G2 phases, presumably by an increase in transcription; activity is decreased by phosphorylation during m phase. Protein levels fall in M phase as a result of decreased synthesis combined with degradation. Activity seems to be negatively regulated by phosphorylation upon entry into mitosis, although N-terminal phosphorylation might also regulate the protein stability via protection from proteolysis or might regulate the subcellular location.,function:May act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase
- 细胞定位:
- Nucleus.
- 组织表达:
- Amygdala,Blood,Epithelium,Human uterus endothel primary cell culture,Placenta,Skin,
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- 产品图片
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using WEE1 (Phospho-Ser53) Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using WEE1 (Phospho-Ser53) Antibody. The picture on the right is blocked with the phospho peptide.