Tra-2α Polyclonal Antibody
- 货号:YT4713
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 简介:
- >>Spliceosome;>>Alcoholic liver disease
- 蛋白名称:
- Transformer-2 protein homolog alpha
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TRA-2 alpha. AA range:221-270
- 特异性:
- Tra-2α Polyclonal Antibody detects endogenous levels of Tra-2α protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TRA2A;Transformer-2 protein homolog alpha;TRA-2 alpha;TRA2-alpha;Transformer-2 protein homolog A
- 背景:
- This gene is a member of the transformer 2 homolog family and encodes a protein with several RRM (RNA recognition motif) domains. This phosphorylated nuclear protein binds to specific RNA sequences and plays a role in the regulation of pre-mRNA splicing. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013],
- 功能:
- function:Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.,PTM:Phosphorylated in the RS domains.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Binds to A3 enhancer proteins SRp75, SRp55, SRp40 and SRp30.,
- 组织表达:
- Cervix carcinoma,Epithelium,Hepatocyte,Lung,Testis,Uterus,
- Western Blot analysis of various cells using Tra-2α Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of HeLa cells, using TRA-2 alpha Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRA-2 alpha Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HUVEC cells, using TRA-2 alpha Antibody. The lane on the right is blocked with the synthesized peptide.