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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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TBX2 Polyclonal Antibody
- 货号:YT4568
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- T-box transcription factor TBX2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TBX2. AA range:251-300
- 特异性:
- TBX2 Polyclonal Antibody detects endogenous levels of TBX2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TBX2;T-box transcription factor TBX2;T-box protein 2
- 背景:
- This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product is the human homolog of mouse Tbx2, and shares strong sequence similarity with Drosophila omb protein. Expression studies indicate that this gene may have a potential role in tumorigenesis as an immortalizing agent. Transcript heterogeneity due to alternative polyadenylation has been noted for this gene. [provided by RefSeq, Jul 2008],
- 功能:
- function:Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.,similarity:Contains 1 T-box DNA-binding domain.,tissue specificity:Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary.,
- 组织表达:
- Expressed primarily in adult in kidney, lung, and placenta. Weak expression in heart and ovary.
- Western Blot analysis of various cells using TBX2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from HeLa cells, using TBX2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).