TAF I p48 Polyclonal Antibody
- 货号：YT4525
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat
- 蛋白名称：
- TATA box-binding protein-associated factor RNA polymerase I subunit A
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human TAF1A. AA range:301-350
- 特异性：
- TAF I p48 Polyclonal Antibody detects endogenous levels of TAF I p48 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- TAF1A;TATA box-binding protein-associated factor RNA polymerase I subunit A;RNA polymerase I-specific TBP-associated factor 48 kDa;TAFI48;TATA box-binding protein-associated factor 1A;TBP-associated factor 1A;Transcription factor SL1;
- 背景：
- This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq, Jan 2011],
- 功能：
- function:Component of the transcription factor SL1/TIFIB complex involved in the assembly of the PIC (preinitiation complex).,subunit:Component of the transcription factor SL1/TIFIB complex which is composed of TBP and at least TAF1A, TAF1B and TAF1C. In the complex, interacts directly with TBP, TAF1B and TAF1C. Interaction of the SL1/TIFIB subunits with TBP excludes interaction with the transcription factor IID (TFIID) subunits. This mutually exclusive binding directs the formation of promoter and RNA polymerase-selective TBP-TAF complexes.,
- Western blot analysis of lysates from HeLa cells, using TAF1A Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
