SOCS-2 Polyclonal Antibody
- 货号：YT4363
- 应用：WB;IHC
- 种属：Human;Mouse;Rat
- 简介：
- >>JAK-STAT signaling pathway;>>Insulin signaling pathway;>>Prolactin signaling pathway;>>Type II diabetes mellitus;>>Growth hormone synthesis, secretion and action
- 蛋白名称：
- Suppressor of cytokine signaling 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human SOCS-2. AA range:18-67
- 特异性：
- SOCS-2 Polyclonal Antibody detects endogenous levels of SOCS-2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SOCS2;CIS2;SSI2;STATI2;Suppressor of cytokine signaling 2;SOCS-2;Cytokine-inducible SH2 protein 2;CIS-2;STAT-induced STAT inhibitor 2;SSI-2
- 背景：
- This gene encodes a member of the suppressor of cytokine signaling (SOCS) family. SOCS family members are cytokine-inducible negative regulators of cytokine receptor signaling via the Janus kinase/signal transducer and activation of transcription pathway (the JAK/STAT pathway). SOCS family proteins interact with major molecules of signaling complexes to block further signal transduction, in part, by proteasomal depletion of receptors or signal-transducing proteins via ubiquitination. The expression of this gene can be induced by a subset of cytokines, including erythropoietin, GM-CSF, IL10, interferon (IFN)-gamma and by cytokine receptors such as growth horomone receptor. The protein encoded by this gene interacts with the cytoplasmic domain of insulin-like growth factor-1 receptor (IGF1R) and is thought to be involved in the regulation of IGF1R mediated cell signaling. This gene has
- 功能：
- domain:The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.,function:SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.,induction:By a subset of cytokines, including erythropoietin and granulocyte-macrophage colony stimulating factor (GM-CSF).,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SOCS box domain.,subunit:Interacts with IGF1 receptor, prolactin receptor and growth h
- 组织表达：
- High expression in heart, placenta, lung, kidney and prostate. Predominantly expressed in pulmonary epithelia cells, specifically type II pneumocytes.
- Western Blot analysis of various cells using SOCS-2 Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysate from Jurkat cells, using SOCS-2 antibody.
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
