PFK-2 liv/tes Polyclonal Antibody
- 货号:YT3684
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse;Rat
- 简介:
- >>Fructose and mannose metabolism;>>Metabolic pathways;>>AMPK signaling pathway;>>Glucagon signaling pathway
- 蛋白名称:
- 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1/4
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human PFKFB1/4. AA range:331-380
- 特异性:
- PFK-2 liv/tes Polyclonal Antibody detects endogenous levels of PFK-2 liv/tes protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PFKFB1;F6PK;PFRX;6-phosphofructo-2-kinase/fructose-2;6-bisphosphatase 1;6PF-2-K/Fru-2,6-P2ase 1;PFK/FBPase 1;6PF-2-K/Fru-2,6-P2ase liver isozyme;PFKFB4;6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4;6PF-2-K/Fru-2,6-P2ase 4;
- 背景:
- This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012],
- 功能:
- catalytic activity:ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.,catalytic activity:Beta-D-fructose 2,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate.,enzyme regulation:Phosphorylation results in inhibition of the kinase activity.,function:Synthesis and degradation of fructose 2,6-bisphosphate.,similarity:In the C-terminal section; belongs to the phosphoglycerate mutase family.,subunit:Homodimer.,tissue specificity:Liver.,
- 细胞定位:
- cytosol,6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex,
- Western Blot analysis of various cells using PFK-2 liv/tes Polyclonal Antibody
- Western blot analysis of lysates from HeLa, HepG2, COLO205, and 293 cells, using PFKFB1/4 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).