• 靶点：
• p115

• 基因名称：
• USO1

• 蛋白名称：
• General vesicular transport factor p115

• Human Gene Id：
• 8615

• Human Swiss Prot No：
• O60763

• Mouse Swiss Prot No：
• Q9Z1Z0

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human USO1. AA range:913-962

• 特异性：
• p115 Polyclonal Antibody detects endogenous levels of p115 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• USO1;VDP;General vesicular transport factor p115;Protein USO1 homolog;Transcytosis-associated protein;TAP;Vesicle-docking protein

• 实测条带：
• 108kD

• 背景：
• The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],

• 功能：
• domain:Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.,function:General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.,PTM:Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.,similarity:Belongs to the VDP/USO1/EDE1 family.,subcellular location:Recycles between the cytosol and the Golgi apparatus during interphase.,

• 细胞定位：
• Cytoplasm, cytosol . Golgi apparatus membrane ; Peripheral membrane protein . Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. .

• 组织表达：
• Epithelium,Human skeletal muscle,Liver,Uterus,

Protein C‐terminal enzymatic labeling identifies novel caspase cleavages during the apoptosis of multiple myeloma cells induced by kinase inhibition. PROTEOMICS Proteomics. 2016 Jan;16(1):60-69 WB Human OPM2 cell
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