- 靶点：
- p115
- 基因名称：
- USO1
- 蛋白名称：
- General vesicular transport factor p115
- Human Gene Id：
- 8615
- Human Swiss Prot No：
- O60763
- Mouse Swiss Prot No：
- Q9Z1Z0
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human USO1. AA range:913-962
- 特异性：
- p115 Polyclonal Antibody detects endogenous levels of p115 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- USO1;VDP;General vesicular transport factor p115;Protein USO1 homolog;Transcytosis-associated protein;TAP;Vesicle-docking protein
- 实测条带：
- 108kD
- 背景：
- The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
- 功能：
- domain:Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.,function:General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.,PTM:Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.,similarity:Belongs to the VDP/USO1/EDE1 family.,subcellular location:Recycles between the cytosol and the Golgi apparatus during interphase.,
- 细胞定位：
- Cytoplasm, cytosol . Golgi apparatus membrane ; Peripheral membrane protein . Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. .
- 组织表达：
- Epithelium,Human skeletal muscle,Liver,Uterus,
Protein C‐terminal enzymatic labeling identifies novel caspase cleavages during the apoptosis of multiple myeloma cells induced by kinase inhibition. PROTEOMICS Proteomics. 2016 Jan;16(1):60-69 WB Human OPM2 cell
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- 产品图片
- Western Blot analysis of various cells using p115 Polyclonal Antibody diluted at 1:2000
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- Western Blot analysis of K562 cells using p115 Polyclonal Antibody diluted at 1:2000
- Western blot analysis of lysates from MCF-7, HeLa, and Jurkat cells, using USO1 Antibody. The lane on the right is blocked with the synthesized peptide.
