- 靶点：
- MLKL
- 简介：
- >>Necroptosis;>>TNF signaling pathway;>>Salmonella infection
- 基因名称：
- MLKL
- 蛋白名称：
- Mixed lineage kinase domain-like protein
- Human Gene Id：
- 197259
- Human Swiss Prot No：
- Q8NB16
- Mouse Swiss Prot No：
- Q9D2Y4
- 免疫原：
- Synthesized peptide derived from MLKL . at AA range: 40-120
- 特异性：
- MLKL Polyclonal Antibody detects endogenous levels of MLKL protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- MLKL;Mixed lineage kinase domain-like protein
- 实测条带：
- 55kD
- 背景：
- This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],
- 功能：
- domain:The protein kinase domain is predicted to be catalytically inactive.,similarity:Belongs to the protein kinase superfamily.,similarity:Contains 1 protein kinase domain.,
- 细胞定位：
- Cytoplasm . Cell membrane . Nucleus . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (PubMed:24316671). Localizes to the nucleus in response to orthomyxoviruses infection (By similarity). .
- 组织表达：
- Chondrocyte,Leukocyte,Lymph node,
Salmonella pSLT-encoded effector SpvB promotes RIPK3-dependent necroptosis in intestinal epithelial cells Cell Death Discov. 2022 Feb;8(1):1-11. WB Human
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of various cells using MLKL Polyclonal Antibody diluted at 1:500
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
