• 靶点：
• Mcl-1

• 简介：
• >>PI3K-Akt signaling pathway;>>Apoptosis;>>JAK-STAT signaling pathway;>>MicroRNAs in cancer

• 基因名称：
• MCL1

• 蛋白名称：
• Induced myeloid leukemia cell differentiation protein Mcl-1

• Human Gene Id：
• 4170

• Human Swiss Prot No：
• Q07820

• Mouse Gene Id：
• 17210

• Mouse Swiss Prot No：
• P97287

• Rat Gene Id：
• 60430

• Rat Swiss Prot No：
• Q9Z1P3

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human MCL1. AA range:91-140

• 特异性：
• Mcl-1 Polyclonal Antibody detects endogenous levels of Mcl-1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• MCL1;BCL2L3;Induced myeloid leukemia cell differentiation protein Mcl-1;Bcl-2-like protein 3;Bcl2-L-3;Bcl-2-related protein EAT/mcl1;mcl1/EAT

• 实测条带：
• About 40kd in human,39kd in mouse and rat

• 背景：
• This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010],

• 功能：
• function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the abs

• 细胞定位：
• Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.

• 组织表达：
• Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th

Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR‐125b with MCL‐1 by regulating mitochondrial apoptosis pathways in ovarian cancer. Cancer Medicine Cancer Med-Us. 2018 Sep;7(9):4530-4541 WB Human,Mouse xenograft SKOV3 cell, A2780 cell
货号：YT2679

Exosome-derived microRNA-29c Induces Apoptosis of BIU-87 Cells by Down Regulating BCL-2 and MCL-1. Asian Pacific Journal of Cancer Prevention 2014 Apr 30 IHC Human Bladder Cancer tissue
货号：YT2679

1118‐20, an indazole diarylurea compound, inhibits hepatocellular carcinoma HepG2 proliferation and tumour angiogenesis involving Wnt/β‐catenin pathway and receptor tyrosine kinases. JOURNAL OF PHARMACY AND PHARMACOLOGY J Pharm Pharmacol. 2015 Sep;67(10):1393-1405 WB Human 1 : 1000 HepG2 cell，HUVECs
货号：YT2679

1082-39, an analogue of sorafenib, inhibited human cancer cell growth more potently than sorafenib." Biomedicine & Pharmacotherapy 68.3 (2014): 335-341.
货号：YT2679

SL1122-37, a novel derivative of sorafenib, has greater effects than sorafenib on the inhibition of human hepatocellular carcinoma (HCC) growth and prevention of angiogenesis." Bioscience trends 7.5 (2013): 237-244.
货号：YT2679

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