- 靶点：
- Exo1
- 简介：
- >>Mismatch repair
- 基因名称：
- EXO1
- 蛋白名称：
- Exonuclease 1
- Human Gene Id：
- 9156
- Human Swiss Prot No：
- Q9UQ84
- Mouse Gene Id：
- 26909
- Mouse Swiss Prot No：
- Q9QZ11
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human EXO1. AA range:61-110
- 特异性：
- Exo1 Polyclonal Antibody detects endogenous levels of Exo1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度：
- 1 mg/ml
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- EXO1;EXOI;HEX1;Exonuclease 1;hExo1;Exonuclease I;hExoI
- 实测条带：
- 94kD
- 背景：
- This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008],
- 功能：
- cofactor:Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.,developmental stage:Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.,function:5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for
- 细胞定位：
- Nucleus . Colocalizes with PCNA to discrete nuclear foci in S-phase.
- 组织表达：
- Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
Elevated EXO1 expression is associated with breast carcinogenesis and poor prognosis. Annals of Translational Medicine Ann Transl Med. 2021 Jan; 9(2): 135 WB Human 1:2000 Normal tissue， breast cancer tissue MDA-MB-231 cell, SKBR3 cell, MCF-7 cell, T47D cell
货号：YT1646
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of various cells using Exo1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of A549 cells, using EXO1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from A549 cells, using EXO1 Antibody. The lane on the right is blocked with the synthesized peptide.
