EphB2 Polyclonal Antibody
- 货号：YT1586
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Ephrin type-B receptor 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human EPHB2. AA range:991-1040
- 特异性：
- EphB2 Polyclonal Antibody detects endogenous levels of EphB2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- EPHB2;DRT;EPHT3;EPTH3;ERK;HEK5;TYRO5;Ephrin type-B receptor 2;Developmentally-regulated Eph-related tyrosine kinase;ELK-related tyrosine kinase;EPH tyrosine kinase 3;EPH-like kinase 5;EK5;hEK5;Renal carcinoma antigen NY-REN-47
- 背景：
- This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple tr
- 功能：
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in EPHB2 are involved in the development of prostate cancer metastasis to the brain [MIM:603688].,disease:Defects in EPHB2 are involved in the progression of prostate cancer [MIM:176807].,function:Receptor for members of the ephrin-B family. Acts as a tumor suppressor.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:The ligand-activated form interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Binds RASGAP through the juxtamembrane tyrosi
- 细胞定位：
- Cell membrane; Single-pass type I membrane protein. Cell projection, axon . Cell projection, dendrite .
- 组织表达：
- Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
- Western Blot analysis of 293 cells using EphB2 Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from Jurkat cells, using EPHB2 Antibody. The lane on the right is blocked with the synthesized peptide.
