PI3 Kinase Class III (Phospho Ser249) rabbit pAb
- 货号:YP1435
- 应用:WB;ELISA;IHC
- 种属:Human;Rat;Mouse;
- 靶点:
- PI 3 Kinase Class 3
- 简介:
- >>Inositol phosphate metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Autophagy - other;>>Autophagy - animal;>>Phagosome;>>Apelin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Salmonella infection;>>Tuberculosis;>>Kaposi sarcoma-associated herpesvirus infection
- 基因名称:
- PIK3C3 VPS34
- 蛋白名称:
- PI3 Kinase Class III (Ser249)
- Human Gene Id:
- 5289
- Human Swiss Prot No:
- Q8NEB9
- Mouse Gene Id:
- 225326
- Mouse Swiss Prot No:
- Q6PF93
- Rat Gene Id:
- 65052
- Rat Swiss Prot No:
- O88763
- 免疫原:
- Synthesized phosho peptide around human PI3 Kinase Class III (Ser249)
- 特异性:
- This antibody detects endogenous levels of Human PI3 Kinase Class III (phospho-Ser249)
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Phosphatidylinositol 3-kinase catalytic subunit type 3 (PI3-kinase type 3) (PI3K type 3) (PtdIns-3-kinase type 3) (EC 2.7.1.137) (Phosphatidylinositol 3-kinase p100 subunit) (Phosphoinositide-3-kinase class 3) (hVps34)
- 实测条带:
- 100kD
- 功能:
- catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.,cofactor:Manganese.,function:Catalytic subunit of the PI3K complex. Involved in the transport of lysosomal enzyme precursors to lysosomes.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 PI3K/PI4K domain.,subunit:Probably forms a complex with AMBRA1 and BECN1 (By similarity). Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4.,tissue specificity:Ubiquitously expressed, with a highest expression in skeletal muscle.,
- 细胞定位:
- Midbody . Late endosome . Cytoplasmic vesicle, autophagosome . As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (PubMed:14617358). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). .
- 组织表达:
- Ubiquitously expressed, with a highest expression in skeletal muscle.
Zhongfeng Capsules protects against cerebral ischemia–reperfusion injury via mediating the phosphoinositide 3-kinase/Akt and toll-like receptor 4/nuclear factor kappa B signaling pathways by regulating neuronal apoptosis and inflammation WB Rat 1:1000 brain tissue/
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Proteomic Study on the Reproductive Toxicity of Tripterygium Glycosides in Rats WB Rat testis tissue /
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Induction of PI3K/Akt-Mediated Apoptosis in Osteoclasts Is a Key Approach for Buxue Tongluo Pills to Treat Osteonecrosis of the Femoral Head.. Frontiers in Pharmacology Front Pharmacol. 2021 Nov;12:729909-729909 WB Mouse 1:500,1:1000 RAW 264.7 cell
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Fucoidan inhibits lymphangiogenesis by downregulating the expression of VEGFR3 and PROX1 in human lymphatic endothelial cells. Oncotarget Oncotarget. 2016 Jun 21; 7(25): 38025–38035 WB Human HLECs
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An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy Diabet Metab Synd Ob. 2021 Feb;14:601-616 WB Human 1:1000 HepG2 cell
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Aaptamine attenuates the proliferation and progression of non-small cell lung carcinoma. PHARMACEUTICAL BIOLOGY Pharm Biol. 2020;58(1):1044-1054 WB Human A549 cell,H1299 cell
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- Antibody-FAQs
- 产品图片
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
- Western Blot analysis of 1 Jurkat treated with LPS, 2 Jurkat ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000