SOX-2 Monoclonal Antibody
- 货号：YM0594
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
- 蛋白名称：
- Transcription factor SOX-2
- 免疫原：
- Purified recombinant fragment of human SOX-2 expressed in E. Coli.
- 特异性：
- SOX-2 Monoclonal Antibody detects endogenous levels of SOX-2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SOX2;Transcription factor SOX-2
- 背景：
- SRY-box 2(SOX2) Homo sapiens This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008],
- 功能：
- disease:Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.,function:Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.,online information:Sox2 entry,PTM:Sumoylation inhibits bin
- 细胞定位：
- Nucleus speckle . Cytoplasm . Nucleus . Acetylation contributes to its nuclear localization and deacetylation by HDAC3 induces a cytoplasmic delocalization (By similarity). Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH) (By similarity). Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus (By similarity). Nuclear import is facilitated by XPO4, a protein that usually acts as a nuclear export signal receptor (By similarity). .
- 组织表达：
- Fetal brain,Lung,Retina,
- Western Blot analysis using SOX-2 Monoclonal Antibody against HEK293 (1) and SOX2-hIgGFc transfected HEK293 (2) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded lung cancer tissues (left) and esophageal cancer tissues (right) with DAB staining using SOX-2 Monoclonal Antibody.
- Immunofluorescence analysis of NTERA-2 cells using SOX-2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
