Negative Control for mouse IgG3, kappa Primary Antibody

    • 货号:VN0004
    • 应用:IHC;IP;IF;ELISA
    • 种属:Human;Mouse;Rat;Bovin;Pig;Chick;
      • Human Swiss Prot No:
      • /
      • 特异性:
      • 0
      • 组成:
      • PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
      • 来源:
      • Mouse, Monoclonal/IgG3, kappa
      • 稀释:
      • IHC 1:200-1000. IF 1:100-500. ELISA 1:1000-5000
      • 纯化工艺:
      • Protein G
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 细胞定位:
      • No staining
      • 产品图片
      • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD42b mouse mAb (left) and mouse IgG3, kappa monoclonal control (VN004, right).
      • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 14 mouse mAb (YM6031, left) and mouse IgG3, kappa monoclonal control (VN004, right).
      • GAPDH was immunoprecipitated from Hela whole cell lysate with anti-GAPDH (PTR2304) antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG (H + L) antibody. Lane 1 (Input) : Hela whole cell lysate Lane 2 (-) :Mouse monoclonal IgG3 (vn0004, Isotype Control) instead of GAPDH antibody in Hela whole cell lysate. Lane 3 (+) : GAPDH antibody IP in Hela whole cell lysate
      • GAPDH was immunoprecipitated from SW480 whole cell lysate with anti-GAPDH (PTR2304) antibody. Western blot was performed on the immunoprecipitate using anti-GAPDH antibody, and followed by the HRP-conjugated Goat anti-Mouse IgG (H + L) antibody. Lane 1 (Input) : SW480 whole cell lysate Lane 2 (-) : Mouse monoclonal IgG3 (vn0004, Isotype Control) instead of GAPDH antibody in SW480 whole cell lysate. Lane 3 (+) : GAPDH antibody IP in SW480 whole cell lysate