mCherry-Tag(PNF001) Nb-FC recombinant antibody
- Catalog No.:YA0648
- Applications:WB
- Reactivity:Species independent
- Target:
- mCherry-Tag
- Immunogen:
- Recombinant Protein of mCherry-Tag
- Specificity:
- The antibody detects mCherry tag fusion proteins.
- Formulation:
- Phosphate-buffered solution
- Source:
- Camel, chimeric fusion of Nanobody (VHH) and mouse IgG1 Fc domain , recombinantly produced from 293F cell
- Dilution:
- WB 1:1000-5000
- Purification:
- Recombinant Expression and Affinity purified
- Storage Stability:
- -15°C to -25°C/1 year(Avoid freeze / thaw cycles)
- Other Name:
- mCherry;mCherry Tag;
- Background:
- mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
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- Antibody-FAQs
- Products Images
- Whole extracts were separated by 10% SDS-PAGE, and the membrane was blotted with anti-mCherry-Tag(PNF001)antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody. Lane 1: BL21 transfected with an empty expression vector Lane 2: BL21 transfected with a mCherry tagged protein Predicted band size: 38kDa Observed band size: 38kDa