Trk A Polyclonal Antibody

    • Catalog No.:YT4742
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • Trk A
      • Fields:
      • >>MAPK signaling pathway;>>Ras signaling pathway;>>Calcium signaling pathway;>>PI3K-Akt signaling pathway;>>Apoptosis;>>Neurotrophin signaling pathway;>>Inflammatory mediator regulation of TRP channels;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Thyroid cancer;>>Central carbon metabolism in cancer
      • Gene Name:
      • NTRK1
      • Protein Name:
      • High affinity nerve growth factor receptor
      • Human Gene Id:
      • 4914
      • Human Swiss Prot No:
      • P04629
      • Mouse Swiss Prot No:
      • Q3UFB7
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Trk A. AA range:666-715
      • Specificity:
      • Trk A Polyclonal Antibody detects endogenous levels of Trk A protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:5000. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NTRK1;MTC;TRK;TRKA;High affinity nerve growth factor receptor;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;
      • Observed Band(KD):
      • 140-180kD
      • Background:
      • This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008],
      • Function:
      • alternative products:Both isoforms have similar biological properties,catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein.,disease:Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1; a rearrangement with TPM3 gen
      • Subcellular Location:
      • Cell membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Recycling endosome membrane ; Single-pass type I membrane protein . Rapidly internalized after NGF binding (PubMed:1281417). Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes. .
      • Expression:
      • Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
      • Products Images
      • Western Blot analysis of 293T NIH-3T3 cells using Trk A Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Trk A Antibody. The picture on the right is blocked with the synthesized peptide.