Rb Polyclonal Antibody

    • Catalog No.:YT4022
    • Applications:WB,IHC-p,ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • RB1
      • Protein Name:
      • Retinoblastoma-associated protein
      • Human Gene Id:
      • 5925
      • Human Swiss Prot No:
      • P06400
      • Mouse Swiss Prot No:
      • P13405
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human Retinoblastoma. AA range:581-630
      • Specificity:
      • Rb Polyclonal Antibody detects endogenous levels of Rb protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
      • MolecularWeight(Da):
      • 106159
      • Observed Band(KD):
      • 110
      • Background:
      • RB transcriptional corepressor 1(RB1) Homo sapiens The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008],
      • Function:
      • disease:Defects in RB1 are a cause of bladder cancer [MIM:109800].,disease:Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].,disease:Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated.,function:Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts
      • Subcellular Location:
      • chromatin,nucleus,nucleoplasm,spindle,cyclin/CDK positive transcription elongation factor complex,SWI/SNF complex,PML body,Rb-E2F complex,
      • Expression:
      • Brain,Carcinoma,Cervix,Peripheral blood,Testis,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from Jurkat cells, treated with PMA 125ng/ml 30', using Retinoblastoma Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from COLO205 cells using Retinoblastoma antibody.