Sin1 (Phospho Thr86) rabbit pAb
- Catalog No.:YP1494
- Applications:WB;ELISA;IHC
- Reactivity:Human;Mouse
- Target:
- Sin1
- Fields:
- >>mTOR signaling pathway
- Gene Name:
- MAPKAP1 MIP1 SIN1
- Protein Name:
- Sin1 (Thr86)
- Human Gene Id:
- 79109
- Human Swiss Prot No:
- Q9BPZ7
- Mouse Gene Id:
- 227743
- Mouse Swiss Prot No:
- Q8BKH7
- Rat Gene Id:
- 296648
- Rat Swiss Prot No:
- Q6AYF1
- Immunogen:
- Synthesized phosho peptide around human Sin1 (Thr86)
- Specificity:
- This antibody detects endogenous levels of Human Mouse Sin1 (phospho-Thr86)
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- Purification:
- The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- Target of rapamycin complex 2 subunit MAPKAP1 (TORC2 subunit MAPKAP1) (Mitogen-activated protein kinase 2-associated protein 1) (Stress-activated map kinase-interacting protein 1) (SAPK-interacting protein 1) (mSIN1)
- Observed Band(KD):
- 58kD
- Background:
- This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008],
- Function:
- function:Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAs signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.,similarity:Belongs to the SIN1 family.,subunit:All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2.,tissue specificity:Ubiquitously expressed, with highest levels in heart and skeletal muscle.,
- Subcellular Location:
- Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus.
- Expression:
- Ubiquitously expressed, with highest levels in heart and skeletal muscle.
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- Antibody-FAQs
- Products Images
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).