• Target：
• Trk B

• Fields：
• >>MAPK signaling pathway;>>Ras signaling pathway;>>Calcium signaling pathway;>>PI3K-Akt signaling pathway;>>Neurotrophin signaling pathway;>>Alcoholism

• Gene Name：
• NTRK2 TRKB

• Protein Name：
• Trk B (Phospho-Y817) Polyclonal Antibody

• Human Gene Id：
• 4915

• Human Swiss Prot No：
• Q16620

• Immunogen：
• Synthesized peptide derived from human Trk B (Phospho-Y817)

• Specificity：
• This antibody detects endogenous phospho levels of Trk B (Phospho-Y817) Polyclonal Antibody at Human, Mouse, Rat

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:50-200, WB 1:500-2000. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• BDNF/NT-3 growth factors receptor (EC 2.7.10.1;GP145-TrkB;Trk-B;Neurotrophic tyrosine kinase receptor type 2;TrkB tyrosine kinase;Tropomyosin-related kinase B)

• Observed Band(KD)：
• 145kD

• Background：
• This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],

• Function：
• alternative products:Additional isoforms seem to exist,catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.,PTM:Ligand-mediated auto-phosphorylation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,similarity:Contains 2 LRR (leucine-rich) repeats.,subunit:Exists in a dynamic equ

• Subcellular Location：
• Cell membrane ; Single-pass type I membrane protein . Endosome membrane ; Single-pass type I membrane protein . Early endosome membrane . Cell projection, axon . Cell projection, dendrite . Cytoplasm, perinuclear region . Cell junction, synapse, postsynaptic density . Internalized to endosomes upon ligand-binding. .

• Expression：
• Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain.

Dexamethasone Protects Against Ischaemic Brain Injury via Inhibiting the pAkt Signalling Pathway Through Increasing Hap1. NEUROTOXICITY RESEARCH 2020 Sep 02 WB Mouse 1 : 500 Brain

The SLITRK4-CNPY3 axis promotes liver metastasis of gastric cancer by enhancing the endocytosis and recycling of TrkB in tumour cells CELLULAR ONCOLOGY Li Jun WB Human BGC823 cell,MKN28 cell

Catalpol rescues LPS-induced cognitive impairment via inhibition of NF-Κb-regulated neuroinflammation and up-regulation of TrkB-mediated BDNF secretion in mice JOURNAL OF ETHNOPHARMACOLOGY Weiqing Hu WB Mouse,Rat 1:500 hippocampal tissue PC12 cell

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