PKC θ (phospho Thr538) Polyclonal Antibody

    • Catalog No.:YP0705
    • Applications:WB,IHC-p,IF(paraffin section),ELISA
    • Reactivity:Human,Mouse,Rat
      • Gene Name:
      • PRKCQ
      • Protein Name:
      • Protein kinase C theta type
      • Human Gene Id:
      • 5588
      • Human Swiss Prot No:
      • Q04759
      • Mouse Swiss Prot No:
      • Q02111
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538. AA range:504-553
      • Specificity:
      • Phospho-PKC θ (T538) Polyclonal Antibody detects endogenous levels of PKC θ protein only when phosphorylated at T538.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Rabbit
      • Dilution:
      • Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • PRKCQ; PRKCT; Protein kinase C theta type; nPKC-theta
      • MolecularWeight(Da):
      • 81865
      • Observed Band(KD):
      • 81
      • Background:
      • protein kinase C theta(PRKCQ) Homo sapiens Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.,enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activ
      • Subcellular Location:
      • immunological synapse,intracellular,cytosol,plasma membrane,
      • Expression:
      • Blood,Brain,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunohistochemistry analysis of paraffin-embedded human brain, using PKC thet (Phospho-Thr538) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from Jurkat cells, using PKC thet (Phospho-Thr538) Antibody. The lane on the right is blocked with the phospho peptide.