CD3 mouse mAb(PT2239)

    • Catalog No.:YM6612
    • Applications:IHC-p, WB
    • Reactivity:Human
      • Gene Name:
      • CD3E T3E
      • Protein Name:
      • CD3
      • Human Gene Id:
      • 916
      • Human Swiss Prot No:
      • P07766
      • Immunogen:
      • Synthesized peptide derived from human CD3
      • Specificity:
      • This antibody detects endogenous levels of human CD3. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Mouse/IgG2a, Kappa
      • Dilution:
      • IHC-p 1:100-500, WB 1:500-2000
      • Purification:
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -20°C/1 year
      • Other Name:
      • T-cell surface glycoprotein CD3 epsilon chain (T-cell surface antigen T3/Leu-4 epsilon chain) (CD antigen CD3e)
      • Background:
      • CD3e molecule(CD3E) Homo sapiens The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008],
      • Function:
      • function:The CD3 complex mediates signal transduction.,online information:CD3E mutation db,similarity:Contains 1 Ig-like (immunoglobulin-like) domain.,similarity:Contains 1 ITAM domain.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.,
      • Subcellular Location:
      • Membranous
      • Expression:
      • Blood,T-cell,
      • Products Images
      • Immunohistochemical analysis of paraffin-embedded Diffuse large B-cell Lymphoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Diffuse large B-cell Lymphoma. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Spleen. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Spleen. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Tonsil. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
      • Immunohistochemical analysis of paraffin-embedded Tonsil. 1, Antibody was diluted at 1:200(4°,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).