鼠兔四标五色荧光检测试剂盒（温和型）_ImmunoWay Biotechnology Company |抗体|病理抗体|诊断抗体

鼠兔四标五色荧光检测试剂盒（温和型）

Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an multuple-Fluorescence kit (RS0070, Immunoway). The section was incubated in 6 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. mIHC Antibody Srping Buffer(YS0124)was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).