Total EGFR Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA3092C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • EGFR
    • Human Gene Id：
    • 1956
    • Human Swiss Prot No：
    • P00533
    • Mouse Swiss Prot No：
    • Q01279
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • Epidermal growth factor receptor (EC 2.7.10.1) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
    • Detection Method：
    • Colorimetric
    • Background：
    • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in EGFR are associated with lung cancer [MIM:211980].,function:Isoform 2/truncated isoform may act as an antagonist.,function:Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin.,miscellaneous:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.,online information:EGFR entry,PTM:Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.,PTM:Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds RIPK1. CBL interacts with the autophosphorylated C-terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. Binds MUC1.,tissue specificity:Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.,
    • Function：
    • regulation of cyclin-dependent protein kinase activity, G1/S transition of mitotic cell cycle, MAPKKK cascade, activation of MAPKK activity, mitotic cell cycle, cell morphogenesis, skeletal system development, ossification, cell activation,regulation of protein amino acid phosphorylation, translation, protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process, response to osmotic stress, response to oxidative stress, cell cycle,cell adhesion, cell surface receptor linked signal transduction, enzyme linked receptor protein signaling pathway,transmembrane receptor protein tyrosine kinase signaling pathway, epidermal growth factor receptor signaling pathway, activation of phospholipase C activity, intracellular signaling cascade, protein kinase cascade, regulation of mitotic cell cycle, sensory organ development, salivary gland development, salivary gland
    • Subcellular Location：
    • Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome . Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). .; [Isoform 2]: Secreted.
    • Expression：
    • Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

    Attributes

    • Code：
    • KA3092C
    • Size
    • 96 / well
    • Price$：
    • 330
    • Delivery：
    • 2024-08-03
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