Phospho SYK (Y323) Cell-Based Colorimetric ELISA Kit

  • Catalog No.：KA1338C
  • Applications：ELISA
  • Reactivity：Human;Mouse;Rat
    • Gene Name：
    • SYK
    • Human Gene Id：
    • 6850
    • Human Swiss Prot No：
    • P43405
    • Mouse Swiss Prot No：
    • P48025
    • Storage Stability：
    • 2-8°C/6 months
    • Other Name：
    • Tyrosine-protein kinase SYK (EC 2.7.10.2) (Spleen tyrosine kinase) (p72-Syk)
    • Detection Method：
    • Colorimetric
    • Background：
    • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-348 and Tyr-352. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.,PTM:Autophosphorylated.,PTM:Phosphorylation on Tyr-323 creates a binding site for c-Cbl, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling.,PTM:Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway.,PTM:Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 SH2 domains.,subunit:Interacts with CBL and SLA when it is phosphorylated. The interaction with SLA may link it to CBL, leading to its destruction. Interacts with phosphorylated NFAM1 (By similarity). Interacts with Epstein-Barr virus LMP2A. Interacts through its SH2 domains with the phosphorylated ITAM domain of CD79A which stimulates SYK autophosphorylation and activation. Interacts with FCRL3.,
    • Function：
    • MAPKKK cascade, activation of MAPK activity, cell activation, regulation of cytokine production, serotonin secretion,regulation of protein amino acid phosphorylation, positive regulation of protein amino acid phosphorylation, activation of immune response, immune response-activating cell surface receptor signaling pathway, immune system development, leukocyte differentiation, positive regulation of immune system process, regulation of leukocyte activation, positive regulation of leukocyte activation, regulation of immune effector process, positive regulation of immune effector process, regulation of leukocyte mediated immunity, immune response-activating signal transduction,immune response-regulating signal transduction, immune response-regulating cell surface receptor signaling pathway,regulation of myeloid leukocyte mediated immunity, generation of a signal involved in cell-cell signal
    • Subcellular Location：
    • Cell membrane . Cytoplasm, cytosol .
    • Expression：
    • Widely expressed in hematopoietic cells (at protein level) (PubMed:8163536). Expressed in neutrophils (at protein level) (PubMed:15123770). Within the B-cell compartment, expressed from pro- and pre-B cells to plasma cells (PubMed:8163536).

    Attributes

    • Code：
    • KA1338C
    • Size
    • 96 / well
    • Price$：
    • 470
    • Delivery：
    • 2024-12-10
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