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试剂盒
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免疫组化试剂
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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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DOC2B rabbit pAb
- 货号:YT7602
- 应用:WB
- 种属:Human;Mouse;Rat
- 免疫原:
- Synthesized peptide derived from human DOC2B AA range: 332-382
- 特异性:
- This antibody detects endogenous levels of DOC2B at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2B is expressed ubiquitously and is suggested to be involved in Ca(2+)-dependent intracellular vesicle trafficking in various types of cells. [provided by RefSeq, Jul 2008],
- 功能:
- domain:C2 domain 1 is involved in binding calcium and phospholipids.,function:May be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids.,similarity:Contains 2 C2 domains.,subunit:Interacts with cytoplasmic dynein light chain TCTEL1/TCTEX1.,tissue specificity:Widely expressed with highest levels in brain and kidney.,
- 细胞定位:
- Cytoplasm. Cytoplasmic granule . Cell membrane ; Peripheral membrane protein . Translocates to the plasma membrane in a calcium-dependent manner. .
- 组织表达:
- Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level).
![](/uploadfiles/PIC/YT7602wb-2019.jpg)
- Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°over night