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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CLCB rabbit pAb
- 货号:YT7442
- 应用:WB
- 种属:Human;Mouse;Rat
- 简介:
- >>Lysosome;>>Endocytosis;>>Synaptic vesicle cycle;>>Endocrine and other factor-regulated calcium reabsorption;>>Huntington disease;>>Bacterial invasion of epithelial cells
- 免疫原:
- Synthesized peptide derived from human CLCB AA range: 168-218
- 特异性:
- This antibody detects endogenous levels of CLCB at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008],
- 功能:
- function:Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.,subcellular location:Cytoplasmic face of coated pits and vesicles.,subunit:Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R.,
- 细胞定位:
- Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic face of coated pits and vesicles.
![](/uploadfiles/PIC/YT7442wb-2019.jpg)
- Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night