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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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LMCD1 rabbit pAb
- 货号:YT7409
- 应用:WB;IHC
- 种属:Human;Mouse
- 免疫原:
- Synthesized peptide derived from human LMCD1 AA range: 270-320
- 特异性:
- This antibody detects endogenous levels of LMCD1 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes a member of the LIM-domain family of zinc finger proteins. The encoded protein contains an N-terminal cysteine-rich domain and two C-terminal LIM domains. The presence of LIM domains suggests involvement in protein-protein interactions. The protein may act as a co-regulator of transcription along with other transcription factors. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013],
- 功能:
- similarity:Contains 1 PET domain.,similarity:Contains 2 LIM zinc-binding domains.,subunit:Interacts with beta-dystroglycan.,tissue specificity:Expressed in many tissues with highest abundance in skeletal muscle.,
- 细胞定位:
- Cytoplasm . Nucleus . May shuttle between the cytoplasm and the nucleus. .
- 组织表达:
- Expressed in the heart (at protein level). Expressed in many tissues with highest abundance in skeletal muscle.
![](/uploadfiles/PIC/YT7409wb-2019.jpg)
- Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/21zigongcc.jpg)
- Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).