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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MMS19 rabbit pAb
- 货号:YT7356
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse
- 免疫原:
- Synthesized peptide derived from human MMS19 AA range: 653-703
- 特异性:
- This antibody detects endogenous levels of MMS19 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 功能:
- function:May play a role in nucleotide excision repair (NER) and RNA polymerase II (POL II) transcription by interacting with ERCC2/XPD and ERCC3/XPB helicases, both subunits of NER-transcription factor TFIIH. May also function as a transcriptional coactivator of estrogen receptor (ER). May be involved in regulation of ER activity by bridging TFIIH with ER or may facilitate TFIIH-mediated phosphorylation of ER in specific promoters and cell types.,similarity:Belongs to the MET18/MMS19 family.,similarity:Contains 7 HEAT repeats.,subunit:Interacts with ERCC2/XPD, ERCC3/XPB and NCOA3/RAC3.,tissue specificity:Ubiquitously expressed with higher expression in testis.,
- 细胞定位:
- Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . In mitosis, enriched on centrosomes during prophase, localizes to the spindle during metaphase and surrounds compacted spindle midzone microtubules during telophase. .
- 组织表达:
- Ubiquitously expressed with higher expression in testis.
![](/uploadfiles/PIC/YT7356wb-2019.jpg)
- Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/25feilinaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).