RBM25 rabbit pAb

    • 货号:YT7325
    • 应用:WB;IHC
    • 种属:Human;Mouse
      • 靶点:
      • RBM25
      • 简介:
      • >>Spliceosome
      • 基因名称:
      • RBM25 RNPC7
      • 蛋白名称:
      • RBM25
      • Human Swiss Prot No:
      • P49756
      • Mouse Swiss Prot No:
      • B2RY56
      • 免疫原:
      • Synthesized peptide derived from human RBM25 AA range: 763-813
      • 特异性:
      • This antibody detects endogenous levels of RBM25 at Human/Mouse
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC 1:50-300
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 分子量:
      • 93kD
      • 功能:
      • PTM:Sumoylated.,sequence caution:Contaminating sequence. Potential poly-A sequence.,sequence caution:Translation N-terminally extended.,similarity:Contains 1 PWI domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Detected predominantly in nuclear speckles. Cytoplasmic localization is faint.,subunit:Interacts with SRRM1. Specifically associates with functional splicing complexes, including Sm proteins and U1, U2, U4, U5 and U6 snRNAs. Associates with exon junction complex (EJC) proteins, including APEX1, BAT1, NCBP1, RBM8A and RNPS1. Interaction with NCBP1 is RNA-dependent.,
      • 细胞定位:
      • Nucleus speckle. Cytoplasm. Colocalizes predominantly, with SFRS2 and LUC7L3 splicing factors, in nuclear speckles. Cytoplasmic localization is faint.
      • 产品图片
      • Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°over night
      • Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).