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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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FKB10 rabbit pAb
- 货号:YT7304
- 应用:WB
- 种属:Human;Mouse
- 基因名称:
- FKBP10 FKBP65 PSEC0056
- 免疫原:
- Synthesized peptide derived from human FKB10 AA range: 339-389
- 特异性:
- This antibody detects endogenous levels of FKB10 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.[provided by RefSeq, Nov 2009],
- 功能:
- catalytic activity:Peptidylproline (omega=180) = peptidylproline (omega=0).,enzyme regulation:Inhibited by both FK506 and rapamycin, but not by cyclosporin A.,function:PPIases accelerate the folding of proteins during protein synthesis.,PTM:Glycosylated and phosphorylated.,similarity:Belongs to the FKBP-type PPIase family.,similarity:Contains 2 EF-hand domains.,similarity:Contains 4 PPIase FKBP-type domains.,
- 细胞定位:
- Endoplasmic reticulum lumen .
- Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night