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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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SRGP1 rabbit pAb
- 货号:YT7116
- 应用:WB
- 种属:Human;Mouse
- 基因名称:
- SRGAP1 ARHGAP13 KIAA1304
- 免疫原:
- Synthesized peptide derived from human SRGP1 AA range: 159-209
- 特异性:
- This antibody detects endogenous levels of SRGP1 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene is a GTPase activator, working with the GTPase CDC42 to negatively regulate neuronal migration. The encoded protein interacts with the transmembrane receptor ROBO1 to inactivate CDC42. [provided by RefSeq, Sep 2016],
- 功能:
- function:GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.,sequence caution:Intron retention.,similarity:Contains 1 FCH domain.,similarity:Contains 1 Rho-GAP domain.,similarity:Contains 1 SH3 domain.,subunit:Interacts with ROBO1, CDC42 and RHOA.,tissue specificity:Expressed in brain, lung, kidney, and testis.,
- 组织表达:
- Expressed in brain, lung, kidney, and testis.
![](/uploadfiles/PIC/YT7116wb-2019.jpg)
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night