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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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ALG13 rabbit pAb
- 货号:YT7071
- 应用:WB
- 种属:Human;Mouse;Rat
- 简介:
- >>N-Glycan biosynthesis;>>Various types of N-glycan biosynthesis;>>Metabolic pathways
- 基因名称:
- ALG13 CXorf45 GLT28D1 MDS031
- 免疫原:
- Synthesized peptide derived from human ALG13 AA range: 69-119
- 特异性:
- This antibody detects endogenous levels of ALG13 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009],
- 功能:
- catalytic activity:UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.,function:Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway.,online information:GlycoGene database,similarity:Belongs to the glycosyltransferase 28 family.,subunit:Heterodimer with ALG14 to form a functional enzyme.,
- 细胞定位:
- [Isoform 2]: Endoplasmic reticulum . Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14.
![](/uploadfiles/PIC/YT7071wb-2019.jpg)
- Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°over night