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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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C99L2 rabbit pAb
- 货号:YT7046
- 应用:WB
- 种属:Human;Mouse;Rat
- 简介:
- >>Cell adhesion molecules;>>Leukocyte transendothelial migration
- 基因名称:
- CD99L2 MIC2L1 UNQ1964/PRO4486
- 免疫原:
- Synthesized peptide derived from human C99L2 AA range: 204-254
- 特异性:
- This antibody detects endogenous levels of C99L2 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010],
- 功能:
- function:May function as a homophilic adhesion molecule. Functions in leukocyte-endothelial cell interactions during leukocyte extravasation, and in particular, at the diapedesis step. Does not seem to be involved in docking of leukocytes to the vessel wall or in lymphocyte diapedesis.,PTM:O-glycosylated.,similarity:Belongs to the CD99 family.,tissue specificity:Expressed in brain, heart, lung, liver, spleen, kidney, stomach, small intestine, skeletal muscle, ovary, thymus, testis and uterus. Lower expression seen in thymus. Expressed in E18 uterus and placenta.,
- 细胞定位:
- Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Cell junction .
- 组织表达:
- Expressed in many tissues, with low expression in thymus.
![](/uploadfiles/PIC/YT7046wb-2019.jpg)
- Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night