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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CISY rabbit pAb
- 货号:YT6966
- 应用:WB
- 种属:Human;Mouse;Rat
- 简介:
- >>Citrate cycle (TCA cycle);>>Glyoxylate and dicarboxylate metabolism;>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids
- 免疫原:
- Synthesized peptide derived from human CISY AA range: 272-322
- 特异性:
- This antibody detects endogenous levels of CISY at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA.,miscellaneous:Citrate synthase is found in nearly all cells capable of oxidative metabolism.,online information:Citrate synthase entry,pathway:Carbohydrate metabolism; tricarboxylic acid cycle.,sequence caution:Intron retention.,similarity:Belongs to the citrate synthase family.,subunit:Homodimer.,
- 细胞定位:
- Mitochondrion matrix.
![](/uploadfiles/PIC/YT6966wb-2019.jpg)
- Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night