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- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CMYA5 rabbit pAb
- 货号:YT6956
- 应用:IHC;IF
- 种属:Human;Mouse
- 基因名称:
- CMYA5 C5orf10 DTNBP2 SPRYD2 TRIM76
- 免疫原:
- Synthesized peptide derived from human CMYA5 AA range: 3866-3916
- 特异性:
- This antibody detects endogenous levels of CMYA5 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:50-200. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 功能:
- domain:Amphipathic helix regions act as an anchoring domain for PKA, and appear to be responsible of the interaction between myospryn and PRKAR2A.,induction:Down-regulated in muscle cell lines derived from patients with Duchenne muscular dystrophy (DMD).,sequence caution:Contaminating sequence. Potential poly-A sequence.,sequence caution:Translated as Trp.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 2 fibronectin type-III domains.,subcellular location:Found predominantly at the periphery of the nucleus but also throughout the cell. Localized at the Z-line costamere connection level of the sarcolemma and in lysosomes.,subunit:Interacts with ACTN2, DTNBP1/dysbindin and PRKAR2A (By similarity). Interacts with DES.,tissue specificity:Expressed in skeletal muscle; at a strong level and in heart.,
- 细胞定位:
- Nucleus . Sarcoplasmic reticulum . Cytoplasm . Cytoplasm, perinuclear region . Cytoplasm, myofibril, sarcomere, M line . Found predominantly at the periphery of the nucleus but also throughout the cell. Localized in lysosomes (By similarity). In skeletal muscles, localizes along myofiber periphery, at costameres (By similarity). Predominantly flanks Z-disks (By similarity). Occasionally present at the M-band level. Colocalized with RYR2 in the sarcoplasmic reticulum (By similarity). .
- 组织表达:
- Expressed in skeletal muscle; at a strong level and in heart.
![](/uploadfiles/PIC/30-ihcchangain20220714.jpg)
- Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).