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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PUS10 rabbit pAb
- 货号:YT6833
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse
- 免疫原:
- Synthesized peptide derived from human PUS10 AA range: 425-475
- 特异性:
- This antibody detects endogenous levels of PUS10 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009],
- 功能:
- catalytic activity:tRNA uridine = tRNA pseudouridine.,function:Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Partially unspliced pre-RNA.,similarity:Belongs to the pseudouridine synthase Pus10 family.,
- 细胞定位:
- Nucleus . Cytoplasm . Mitochondrion . Localizes mainly in the nucleus (Probable) (PubMed:31819270). tRNA pseudouridylate synthase activity is restricted to the cytoplasm (PubMed:31819270). Translocates from nucleus to mitochondria during TRAIL-induced apoptosis (PubMed:28981101). .
![](/uploadfiles/PIC/YT6833wb-2019.jpg)
- Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/48gongjingaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).