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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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ADAMTSL4 rabbit pAb
- 货号:YT6831
- 应用:WB
- 种属:Human;Mouse;Rat
- 基因名称:
- ADAMTSL4 TSRC1 PP1396 UNQ2803/PRO34012
- 免疫原:
- Synthesized peptide derived from human ATL4 AA range: 78-128
- 特异性:
- This antibody detects endogenous levels of ATL4 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene is a member of ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs)-like gene family and encodes a protein with seven thrombospondin type 1 repeats. The thrombospondin type 1 repeat domain is found in many proteins with diverse biological functions including cellular adhesion, angiogenesis, and patterning of the developing nervous system. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Sep 2014],
- 功能:
- caution:Although similar to members of the ADAMTS family, it lacks the metalloprotease and disintegrin-like domains which are typical of that family.,function:Positive regulation of apoptosis.,similarity:Contains 1 PLAC domain.,similarity:Contains 6 TSP type-1 domains.,subunit:Interacts with CTSB.,
- 细胞定位:
- Secreted, extracellular space, extracellular matrix . Colocalizes with FMN1 microfibrils in the eye ECM.
- 组织表达:
- Expressed in colon, heart, leukocyte, liver, lung, skeletal muscle, spleen, testis and placenta. Weaker expression in bone marrow, brain tissue, kidney and pancreas. Expression studies in fetal tissues reveal strong expression in heart, kidney, liver, lung and skeletal muscle, but weaker expression in fetal brain and skin.
![](/uploadfiles/PIC/YT6831wb-2019.jpg)
- Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°over night