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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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FHL5 rabbit pAb
- 货号:YT6794
- 应用:WB
- 种属:Human;Mouse;Rat
- 免疫原:
- Synthesized peptide derived from human FHL5 AA range: 129-179
- 特异性:
- This antibody detects endogenous levels of FHL5 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene. Polymorphisms in this gene may be associated with susceptibility for migraine headaches. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Apr 2016],
- 功能:
- function:May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner.,similarity:Contains 4 LIM zinc-binding domains.,subcellular location:Nuclei of round and elongated spermatids.,subunit:Interacts with CREM via the third LIM domain.,tissue specificity:Testis-specific (at protein level).,
- 细胞定位:
- Nucleus . Nuclei of round and elongated spermatids.
- 组织表达:
- Testis-specific (at protein level).
![](/uploadfiles/PIC/YT6794wb-2019.jpg)
- Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°over night