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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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TRI69 rabbit pAb
- 货号:YT6746
- 应用:WB
- 种属:Human;Mouse;Rat
- 基因名称:
- TRIM69 RNF36 HSD-34 HSD34
- 免疫原:
- Synthesized peptide derived from human TRI69 AA range: 202-252
- 特异性:
- This antibody detects endogenous levels of TRI69 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008],
- 功能:
- function:May play a role in apoptosis.,PTM:Phosphorylated. Phosphorylation is necessary for nuclear localization.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with PML.,
- 细胞定位:
- Cytoplasm . Nucleus . Nucleus speckle .
![](/uploadfiles/PIC/YT6746wb-2019.jpg)
- Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°over night