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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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TBCD rabbit pAb
- 货号:YT6655
- 应用:WB
- 种属:Human;Mouse
- 基因名称:
- TBCD KIAA0988 SSD1 TFCD PP1096
- 免疫原:
- Synthesized peptide derived from human TBCD AA range: 419-469
- 特异性:
- This antibody detects endogenous levels of TBCD at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Cofactor D is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. [provided by RefSeq, Jul 2008],
- 功能:
- function:Tubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB).,induction:Down-regulated by shear stress.,similarity:Belongs to the TBCD family.,similarity:Contains 3 HEAT repeats.,subunit:Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.,tissue specificity:Ubiquitously expressed.,
- 细胞定位:
- Cell junction, tight junction . Lateral cell membrane . Cytoplasm . Cell junction, adherens junction . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localized in cell-cell contacts. .
- 组织表达:
- Ubiquitously expressed.
![](/uploadfiles/PIC/YT6655wb-2019.jpg)
- Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night