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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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RHBT2 rabbit pAb
- 货号:YT6490
- 应用:WB;ELISA;IHC
- 种属:Human;Mouse
- 简介:
- >>Ubiquitin mediated proteolysis
- 基因名称:
- RHOBTB2 DBC2 KIAA0717
- 免疫原:
- Synthesized peptide derived from human RHBT2 AA range: 573-623
- 特异性:
- This antibody detects endogenous levels of RHBT2 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011],
- 功能:
- similarity:Belongs to the small GTPase superfamily. Rho family.,similarity:Contains 2 BTB (POZ) domains.,tissue specificity:Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.,
- 细胞定位:
- intracellular,cytosol,plasma membrane,
- 组织表达:
- Ubiquitous, with highest levels in neural tissues. Expression is also detected in fetal lung, heart, and brain.
![](/uploadfiles/PIC/YT6490wb-2019.jpg)
- Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/58xiaochangaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).