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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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ILVBL rabbit pAb
- 货号:YT6407
- 应用:WB
- 种属:Human;Mouse
- 免疫原:
- Synthesized peptide derived from human ILVBL AA range: 87-137
- 特异性:
- This antibody detects endogenous levels of ILVBL at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria, yeast, and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS), which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq, Jul 2008],
- 功能:
- cofactor:Binds 1 magnesium ion per subunit.,cofactor:Binds 1 thiamine pyrophosphate per subunit.,sequence caution:Sequence of unknown origin at the C-terminus.,sequence caution:Translation N-terminally extended.,similarity:Belongs to the TPP enzyme family.,tissue specificity:Expressed in all tissues tested, with highest expression in heart, pancreas and placenta.,
- 细胞定位:
- Endoplasmic reticulum membrane ; Single-pass membrane protein .
- 组织表达:
- Expressed in all tissues tested, with highest expression in heart, pancreas and placenta.
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night